Composite

Part:BBa_K208051

Designed by: USU iGEM 2009   Group: iGEM09_Utah_State   (2009-10-20)

Tet Promoter/RBS/TorA/GFP/Terminator

This is a GFP composite with a TorA signal peptide, promoter, RBS, and terminator. This is in pSB1AK3.

NOTE: The scar between the signal peptide and the protein is not presented correctly in the sequence below. The scar is the Silver-fusion scar (ACTAGA), which allows for in-frame fusion. Additionally, the scars before the signal peptide and after the protein are affected by these parts each having the Silver-fusion prefix and suffix.

Usage and Biology

This part can be used to demonstrate GFP secretion via the Tat pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 735
    Illegal XhoI site found at 636
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None